| Round | Date | StepH | Home | HS | AS | Away | StepA | Pens | Upset | L – H | L- A | Match | S v S | 1 | 2 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| R1 | Sat, Oct 26, 2024 | 3 | AFC Totton | 1 | 0 | Dorchester Town | 3 | 3. SPS | 3. SPS | 3. SPS v 3. SPS | 3 v 3 | 2 | 6 | ||
| R1 | Sat, Oct 26, 2024 | 4 | Barton Rovers | 0 | 3 | Folkestone Invicta | 3 | 4. S1C | 3. IPL | 4. S1C v 3. IPL | 4 v 3 | 6 | 6 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Barwell | 0 | 2 | Sporting Khalsa | 4 | Yes | 3. SPC | 4. NPM | 3. SPC v 4. NPM | 3 v 4 | 16 | 4 | |
| R1 | Sat, Oct 26, 2024 | 3 | Basford United | 1 | 1 | Anstey Nomads | 4 | 5-3 | 3. NPL | 4. NPM | 3. NPL v 4. NPM | 3 v 4 | 14 | 7 | |
| R1 | Sat, Oct 26, 2024 | 3 | Biggleswade Town | 2 | 0 | Potters Bar Town | 3 | 3. SPC | 3. IPL | 3. SPC v 3. IPL | 3 v 3 | 22 | 13 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Bishops Stortford | 1 | 6 | Felixstowe & Walton United | 4 | Yes | 3. SPC | 4. ILN | 3. SPC v 4. ILN | 3 v 4 | 12 | 2 | |
| R1 | Sat, Oct 26, 2024 | 3 | Bowers & Pitsea | 0 | 1 | Chatham Town | 3 | 3. IPL | 3. IPL | 3. IPL v 3. IPL | 3 v 3 | 21 | 10 | ||
| R1 | Sat, Oct 26, 2024 | 4 | Brentwood Town | 4 | 3 | Cray Valley (Paper Mills) | 3 | Yes | 4. ILN | 3. IPL | 4. ILN v 3. IPL | 4 v 3 | 7 | 2 | |
| R1 | Sat, Oct 26, 2024 | 4 | Bristol Manor Farm | 1 | 1 | Havant & Waterlooville | 3 | 2-4 | 4. S1S | 3. SPS | 4. S1S v 3. SPS | 4 v 3 | 7 | 4 | |
| R1 | Sat, Oct 26, 2024 | 4 | Cambridge City | 1 | 2 | Royston Town | 3 | 4. ILN | 3. SPC | 4. ILN v 3. SPC | 4 v 3 | 16 | 17 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Canvey Island | 0 | 2 | Horsham | 3 | 3. IPL | 3. IPL | 3. IPL v 3. IPL | 3 v 3 | 11 | 14 | ||
| R1 | Sat, Oct 26, 2024 | 4 | Chasetown | 1 | 1 | Belper Town | 4 | 5-4 | 4. NPW | 4. NPE | 4. NPW v 4. NPE | 4 v 4 | 6 | 3 | |
| R1 | Sat, Oct 26, 2024 | 4 | Clitheroe | 1 | 4 | Gainsborough Trinity | 3 | 4. NPW | 3. NPL | 4. NPW v 3. NPL | 4 v 3 | 17 | 20 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Dover Athletic | 0 | 2 | Sittingbourne | 4 | Yes | 3. IPL | 4. ISE | 3. IPL v 4. ISE | 3 v 4 | 1 | 2 | |
| R1 | Sat, Oct 26, 2024 | 3 | Dulwich Hamlet | 1 | 1 | Hashtag United | 3 | 4-5 | 3. IPL | 3. IPL | 3. IPL v 3. IPL | 3 v 3 | 12 | 16 | |
| R1 | Sat, Oct 26, 2024 | 3 | Gloucester City | 2 | 3 | Coleshill Town | 4 | Yes | 3. SPS | 4. NPM | 3. SPS v 4. NPM | 3 v 4 | 3 | 13 | |
| R1 | Sat, Oct 26, 2024 | 3 | Gosport Borough | 5 | 1 | Taunton Town | 3 | 3. SPS | 3. SPS | 3. SPS v 3. SPS | 3 v 3 | 13 | 14 | ||
| R1 | Sat, Oct 26, 2024 | 4 | Hadley | 4 | 3 | Hastings United | 3 | Yes | 4. S1C | 3. IPL | 4. S1C v 3. IPL | 4 v 3 | 4 | 19 | |
| R1 | Sat, Oct 26, 2024 | 3 | Halesowen Town | 4 | 1 | Congleton Town | 4 | 3. SPC | 4. NPW | 3. SPC v 4. NPW | 3 v 4 | 2 | 4 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Hanwell Town | 5 | 0 | Hendon | 3 | 3. SPS | 3. IPL | 3. SPS v 3. IPL | 3 v 3 | 10 | 7 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Hungerford Town | 2 | 3 | Yate Town | 4 | Yes | 3. SPS | 4. S1S | 3. SPS v 4. S1S | 3 v 4 | 19 | 1 | |
| R1 | Sat, Oct 26, 2024 | 3 | Lewes | 2 | 2 | Ashford United | 4 | 8-7 | 3. IPL | 4. ISE | 3. IPL v 4. ISE | 3 v 4 | 5 | 11 | |
| R1 | Sat, Oct 26, 2024 | 3 | Macclesfield | 7 | 1 | Ashton United | 3 | 3. NPL | 3. NPL | 3. NPL v 3. NPL | 3 v 3 | 1 | 5 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Mickleover | 1 | 2 | AFC Telford United | 3 | 3. NPL | 3. SPC | 3. NPL v 3. SPC | 3 v 3 | 17 | 3 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Plymouth Parkway | 0 | 4 | Chichester City | 3 | 3. SPS | 3. IPL | 3. SPS v 3. IPL | 3 v 3 | 20 | 15 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Stockton Town | 2 | 1 | Newton Aycliffe | 4 | 3. NPL | 4. NPE | 3. NPL v 4. NPE | 3 v 4 | 6 | 7 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Stourbridge | 0 | 1 | Alvechurch | 3 | 3. SPC | 3. SPC | 3. SPC v 3. SPC | 3 v 3 | 7 | 13 | ||
| R1 | Sat, Oct 26, 2024 | 4 | Tavistock AFC | 0 | 4 | Winchester City | 3 | 4. S1S | 3. SPS | 4. S1S v 3. SPS | 4 v 3 | 15 | 18 | ||
| R1 | Sat, Oct 26, 2024 | 4 | Uxbridge | 0 | 3 | Chertsey Town | 3 | 4. ISC | 3. SPS | 4. ISC v 3. SPS | 4 v 3 | 6 | 8 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Warrington Rylands 1906 | 4 | 0 | Ossett United | 4 | 3. NPL | 4. NPE | 3. NPL v 4. NPE | 3 v 4 | 19 | 21 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Workington | 2 | 3 | Bamber Bridge | 3 | 3. NPL | 3. NPL | 3. NPL v 3. NPL | 3 v 3 | 12 | 15 | ||
| R1 | Sat, Oct 26, 2024 | 3 | Worksop Town | 2 | 0 | FC United of Manchester | 3 | 3. NPL | 3. NPL | 3. NPL v 3. NPL | 3 v 3 | 2 | 18 |