| Round | Date | Step H | Home | HS | – | AS | Away | Step A |
|---|---|---|---|---|---|---|---|---|
| Round 1 | Sun, Oct 19, 2025 | Borrowash | 0 | – | 0 | Long Eaton United | 6 | |
| Round 1 | Sun, Oct 19, 2025 | 6 | Buxton | H | – | W | Matlock Town | |
| Round 1 | Sun, Oct 19, 2025 | Chesterfield Development | 3 | – | 8 | Melbourne Dynamo | ||
| Round 1 | Sun, Oct 19, 2025 | Codnor Sports Vixens | W | – | A | Sleetmoor United | ||
| Round 1 | Sun, Oct 19, 2025 | Gresley Rovers | W | – | A | Staveley Miners Welfare | ||
| Round 1 | Sun, Oct 19, 2025 | Heanor | 3 | – | 1 | Hatton United | ||
| Round 1 | Sun, Oct 19, 2025 | Staveley Miners Welfare Reserves | 2 | – | 0 | Stanton Ilkeston | ||
| Round 2 | Sun, Nov 16, 2025 | Ashbourne | – | Belper Town Reserves | ||||
| Round 2 | Sun, Nov 16, 2025 | Bakewell Town | 3 | – | 2 | Draycott Victoria | ||
| Round 2 | Sun, Nov 16, 2025 | Buxton | 0 | 0p | Hilton Harriers | |||
| Round 2 | Sun, Nov 16, 2025 | Buxton Juniors | 2 | 3 | Wirksworth Colts | |||
| Round 2 | Sun, Nov 16, 2025 | Chesterfield | 9 | 0 | Staveley Miners Welfare Reserves | |||
| Round 2 | Sun, Nov 16, 2025 | Gresley Rovers | – | Melbourne Dynamo Reserves | ||||
| Round 2 | Sun, Nov 16, 2025 | Long Eaton United Development | 1 | – | 2 | Chesterfield Youth | ||
| Round 2 | Sun, Nov 16, 2025 | Melbourne Dynamo | H | W | Allenton United | |||
| Round 2 | Sun, Nov 16, 2025 | Pride Park | 4 | 2 | Belper Town | 6 | ||
| Round 2 | Sun, Nov 16, 2025 | Sleetmoor United | 0p | 0 | Courage FC | |||
| Round 2 | Derwent | Staveley Miners Welfare | ||||||
| Round 2 | Duffield Dynamo | – | Long Eaton United | 6 | ||||
| Round 2 | Heanor | Pride Park Development | ||||||
| Round 2 | Stapenhill | – | Glossop North End | |||||
| Round 3 | ||||||||
| Round 3 | ||||||||
| Round 3 | ||||||||
| Round 3 | ||||||||
| Round 3 | ||||||||
| Round 3 | ||||||||
| Round 3 | ||||||||
| Round 3 | ||||||||
| Quarter Final | ||||||||
| Quarter Final | ||||||||
| Quarter Final | ||||||||
| Quarter Final | ||||||||
| Semi Final | ||||||||
| Semi Final | ||||||||
| Final |
