| StepH | Home | HS | AS | Away | StepA | Pens | Upset |
|---|---|---|---|---|---|---|---|
| Mon, Aug 19, 2024 | |||||||
| 4 | Melksham Town | 4 | 1 | Shaftesbury | 4 | ||
| Tue, Aug 20, 2024 | |||||||
| 4 | North Ferriby | 2 | 4 | Newcastle Blue Star | 5 | Yes | |
| 4 | Bishop Auckland | 1 | 2 | Ashington | 4 | ||
| 4 | Wythenshawe Town | 3 | 1 | Runcorn Linnets | 4 | ||
| 4 | Witton Albion | 3 | 1 | South Liverpool | 5 | ||
| 5 | Northwich Victoria | 2 | 4 | Barnoldswick Town | 5 | ||
| 4 | Sheffield | 3 | 2 | Clitheroe | 4 | ||
| 4 | Newcastle Town | 2 | 0 | Westfields | 5 | ||
| 4 | Sporting Khalsa | 2 | 0 | Gresley Rovers | 5 | ||
| 4 | Loughborough Students | 4 | 0 | Deeping Rangers | 5 | ||
| 4 | Felixstowe & Walton United | 4 | 0 | Bury Town | 4 | ||
| 5 | Harleston Town | 4 | 0 | Soham Town Rangers | 5 | ||
| 4 | Heybridge Swifts | 1 | 1 | Leverstock Green | 4 | 3-4 | |
| 5 | Nailsea & Tickenham | 1 | 0 | Reading City | 5 | ||
| 4 | Uxbridge | 1 | 2 | Ashford Town (Middlesex) | 4 | ||
| 4 | Ashford United | 3 | 1 | Harrow Borough | 4 | ||
| 4 | Rayners Lane | 1 | 2 | Herne Bay | 4 | ||
| 4 | Three Bridges | 3 | 1 | Saltdean United | 5 | ||
| 5 | Fleet Town | 4 | 1 | Wincanton Town | 5 | ||
| 5 | Wellington AFC | 0 | 2 | Bideford | 4 | ||
| Wed, Aug 21, 2024 | |||||||
| 4 | Heaton Stannington | 2 | 3 | Garforth Town | 4 | ||
| 5 | Eccleshill United | 1 | 3 | Mossley | 4 | ||
| 4 | Willand Rovers | 1 | 0 | Mousehole AFC | 4 | ||
| 4 | Dunston UTS | 3 | 0 | Whickham | 5 | ||
| Tue, Aug 27, 2024 | |||||||
| 5 | Millbrook | 1 | 3 | AFC Stoneham | 5 | ||