| Round | Date | Step H | Home | HS | – | AS | Away | Step A |
|---|---|---|---|---|---|---|---|---|
| 1st Round | Sun, Oct 6, 2024 | 6 | Thetford Town | 3 | – | 1 | Toby | 6 |
| 1st Round | Sun, Oct 6, 2024 | 6 | Frontiers | 0 | – | 2 | Biggleswade United | 6 |
| 1st Round | Sun, Oct 6, 2024 | 6 | Lawford | w/o | – | Cheshunt | 6 | |
| 2nd Round | Sun, Oct 27, 2024 | 6 | AFC Dunstable | 1 | – | 4 | Stevenage | 5 |
| 2nd Round | Sun, Oct 27, 2024 | 5 | Cambridge City | 0 | – | 3 | Real Bedford Reserves | 6 |
| 2nd Round | Sun, Oct 27, 2024 | 6 | Hutton | 0 | – | 3 | St Albans City | 5 |
| 2nd Round | Sun, Oct 27, 2024 | 5 | Luton Town | 5 | – | 0 | Hertford Town | 6 |
| 2nd Round | Sun, Oct 27, 2024 | 6 | Mulbarton Wanderers | 0 | – | 7 | Bowers & Pitsea | 5 |
| 2nd Round | Sun, Oct 27, 2024 | 5 | Royston Town | 4 | – | 0 | Colney Heath | 6 |
| 2nd Round | Sun, Oct 27, 2024 | 5 | Wroxham | 3 | – | 1 | Chelmsford City | 6 |
| 2nd Round | Sun, Nov 3, 2024 | 6 | Biggleswade United | 4 | 1 | Romford Town | 6 | |
| 2nd Round | Sun, Nov 3, 2024 | 5 | Enfield Town | 4 | – | 0 | Histon | 6 |
| 2nd Round | Sun, Nov 3, 2024 | 5 | Haringey Borough | 3 | – | 2 | Harpenden Town | 6 |
| 2nd Round | Sun, Nov 3, 2024 | 6 | Kings Lynn Town | 0 | – | 6 | Thetford Town | 6 |
| 2nd Round | Sun, Nov 3, 2024 | 6 | Lawford | 3 | – | 4 | Stanway Pegasus | 6 |
| 2nd Round | Sun, Nov 3, 2024 | 6 | Newmarket Town | 3 | – | 2 | Wootton Blue Cross Lionesses | 6 |
| 2nd Round | Sun, Nov 3, 2024 | 6 | Southend United | 0 | – | 5 | Watford Ladies Development | 5 |
| 2nd Round | Sun, Nov 3, 2024 | 6 | Whittlesey Athletic | 2 | – | 5 | Needham Market | 5 |
| 2nd Round | Sun, Nov 3, 2024 | 6 | Wormley Rovers | 1 | – | 2 | Dussindale & Hellesdon Rovers | 5 |
| 3rd Round | Sun, Dec 1, 2024 | 5 | Bowers & Pitsea | – | Royston Town | 5 | ||
| 3rd Round | Sun, Dec 1, 2024 | 5 | Enfield Town | – | Dussindale & Hellesdon Rovers | 5 | ||
| 3rd Round | Sun, Dec 1, 2024 | 5 | Haringey Borough | – | Wroxham | 5 | ||
| 3rd Round | Sun, Dec 1, 2024 | 6 | Real Bedford Reserves | – | Newmarket Town | 6 | ||
| 3rd Round | Sun, Dec 1, 2024 | 6 | Stanway Pegasus | – | Newmarket Town | 6 | ||
| 3rd Round | Sun, Dec 1, 2024 | 6 | Thetford Town | – | Biggleswade United | 6 | ||
| 3rd Round | Sun, Dec 1, 2024 | 5 | Watford Ladies Development | – | Luton Town | 5 | ||
| 3rd Round | Sun, Dec 8, 2024 | 5 | St Albans City | – | Stevenage | 5 | ||
| Quarter Finals | ||||||||
| Quarter Finals | ||||||||
| Quarter Finals | ||||||||
| Quarter Finals | ||||||||
| Semi Finals | ||||||||
| Semi Finals | ||||||||
| Final |