| Preliminary Round |
| Fri Aug 22, 2025 |
| 4 | Egham Town | 0 | v | 2 | Margate | 4 | | | 4. ISC | 4. ISE |
| Sat Aug 23, 2025 |
| 4 | Garforth Town | 3 | v | 0 | Ashington | 4 | | | 4. NPE | 4. NPE |
| 4 | Consett | 1 | v | 1 | Bishop Auckland | 4 | 5-4 | | 4. NPE | 4. NPE |
| 4 | Sporting Khalsa | 1 | v | 1 | Wellingborough Town | 4 | 4-3 | | 4. NPW | 4. NPM |
| 4 | Sutton Coldfield Town | 3 | v | 2 | Rugby Borough | 4 | | | 4. NPM | 4. NPM |
| 4 | Brightlingsea Regent | 3 | v | 1 | Barton Rovers | 4 | | | 4. ILN | 4. S1C |
| 4 | Heybridge Swifts | 1 | v | 1 | Wroxham | 4 | 6-7 | | 4. ILN | 4. ILN |
| 4 | Brantham Athletic | 0 | v | 4 | Grays Athletic | 4 | | | 4. ILN | 4. ILN |
| 4 | Leighton Town | 2 | v | 0 | Gorleston | 4 | | | 4. S1C | 4. ILN |
| 4 | Northwood | 3 | v | 1 | Hanworth Villa | 4 | | | 4. S1C | 4. ISC |
| 4 | AFC Croydon Athletic | 1 | v | 3 | AFC Whyteleafe | 4 | | | 4. ISE | 4. ISE |
| 4 | Sheppey United | 1 | v | 1 | Hassocks | 4 | 0-3 | | 4. ISE | 4. ISE |
| 4 | Metropolitan Police | 1 | v | 1 | Hartley Wintney | 4 | 4-2 | | 4. ISC | 4. ISC |
| 4 | Marlow | 0 | v | 3 | Three Bridges | 4 | | | 4. S1C | 4. ISE |
| 4 | Bashley | 0 | v | 3 | Frome Town | 4 | | | 4. S1S | 4. S1S |
| 4 | Westbury United | 1 | v | 1 | Falmouth Town | 4 | 7-8 | | 4. S1S | 4. S1S |
| 1st Qualifying Round |
| Fri Sep 5, 2025 |
| 4 | Bootle | 2 | v | 3 | Witton Albion | 4 | | | 4. NPW | 4. NPW |
| 4 | Heaton Stannington | 2 | v | 0 | Emley AFC | 4 | | | 4. NPE | 4. NPE |
| 4 | Enfield | 1 | v | 0 | Wroxham | 4 | | | 4. S1C | 4. ILN |
| 4 | Ascot United | 2 | v | 0 | Three Bridges | 4 | | | 4. ISC | 4. ISE |
| Sat Sep 6, 2025 |
| 4 | Newton Aycliffe | 3 | v | 0 | Blyth Town | 4 | | | 4. NPE | 4. NPE |
| 4 | Dunston UTS | 4 | v | 1 | Brighouse Town | 4 | | | 4. NPE | 4. NPE |
| 4 | Stalybridge Celtic | 2 | v | 0 | Trafford | 4 | | | 4. NPW | 4. NPW |
| 4 | Ossett United | 1 | v | 3 | Nantwich Town | 4 | | | 4. NPE | 4. NPW |
| 4 | Consett | 4 | v | 0 | Hallam | 4 | | | 4. NPE | 4. NPE |
| 4 | North Ferriby | 1 | v | 0 | Blyth Spartans | 4 | | | 4. NPE | 4. NPE |
| 4 | Silsden | 1 | v | 0 | Atherton Collieries | 4 | | | 4. NPE | 4. NPW |
| 4 | Congleton Town | 3 | v | 1 | Vauxhall Motors | 4 | | | 4. NPW | 4. NPW |
| 4 | Bradford (Park Avenue) | 1 | v | 1 | Redcar Athletic | 4 | 6-5 | | 4. NPE | 4. NPE |
| 4 | Bury | 3 | v | 1 | Wythenshawe Town | 4 | | | 4. NPW | 4. NPW |
| 4 | Runcorn Linnets | 1 | v | 1 | Garforth Town | 4 | 4-1 | | 4. NPW | 4. NPE |
| 4 | Avro | 1 | v | 3 | Clitheroe | 4 | | | 4. NPW | 4. NPW |
| 4 | Bridlington Town | 1 | v | 0 | Mossley | 4 | | | 4. NPE | 4. NPW |
| 4 | Long Eaton United | 2 | v | 2 | Carlton Town | 4 | | | 4. NPM | 4. NPM |
| 4 | Belper Town | 2 | v | 1 | Newcastle Town | 4 | | | 4. NPM | 4. NPW |
| 4 | Coventry Sphinx | 0 | v | 2 | Lincoln United | 4 | | | 4. NPM | 4. NPE |
| 4 | Stafford Rangers | 1 | v | 0 | Boldmere St Michaels | 4 | | | 4. NPW | 4. NPM |
| 4 | Rugby Town | 4 | v | 1 | Racing Club Warwick | 4 | | | 4. NPM | 4. NPM |
| 4 | Sutton Coldfield Town | 3 | v | 2 | Kidsgrove Athletic | 4 | | | 4. NPM | 4. NPW |
| 4 | Shifnal Town | 1 | v | 0 | Chasetown | 4 | | | 4. NPW | 4. NPW |
| 4 | Mickleover | 2 | v | 3 | Basford United | 4 | | | 4. NPM | 4. NPM |
| 4 | Sporting Club Inkberrow | 0 | v | 0 | St. Neots Town | 4 | 3-1 | | 4. S1S | 4. NPM |
| 4 | Bedworth United | 0 | v | 0 | Darlaston Town | 4 | 7-6 | | 4. NPM | 4. NPW |
| 4 | Lichfield City | 1 | v | 2 | Corby Town | 4 | | | 4. NPM | 4. NPM |
| 4 | Bourne Town | 0 | v | 2 | AFC Rushden & Diamonds | 4 | | | 4. NPM | 4. NPM |
| 4 | Coleshill Town | 1 | v | 0 | Sporting Khalsa | 4 | | | 4. NPM | 4. NPW |
| 4 | Malvern Town | 5 | v | 1 | Loughborough Students | 4 | | | 4. S1S | 4. NPM |
| 4 | Shepshed Dynamo | 0 | v | 4 | Anstey Nomads | 4 | | | 4. NPM | 4. NPM |
| 4 | Matlock Town | 3 | v | 0 | Grimsby Borough | 4 | | | 4. NPE | 4. NPE |
| 4 | Ware | 1 | v | 5 | Hadley | 4 | | | 4. S1C | 4. S1C |
| 4 | Hertford Town | 1 | v | 3 | Felixstowe & Walton United | 4 | | | 4. S1C | 4. ILN |
| 4 | Maldon & Tiptree | 0 | v | 1 | AFC Dunstable | 4 | | | 4. ILN | 4. S1C |
| 4 | Takeley | 0 | v | 2 | Welwyn Garden City | 4 | | | 4. ILN | 4. S1C |
| 4 | Stotfold | 0 | v | 2 | Mildenhall Town | 4 | | | 4. S1C | 4. ILN |
| 4 | Biggleswade Town | 1 | v | 3 | Hitchin Town | 4 | | | 4. S1C | 4. S1C |
| 4 | Grays Athletic | 2 | v | 1 | Milton Keynes Irish | 4 | | | 4. ILN | 4. S1C |
| 4 | Witham Town | 2 | v | 2 | Newmarket Town | 4 | 4-1 | | 4. ILN | 4. ILN |
| 4 | Stanway Rovers | 4 | v | 0 | Aylesbury United | 4 | | | 4. ILN | 4. S1C |
| 4 | Walthamstow | 3 | v | 2 | Downham Town | 4 | | | 4. ILN | 4. ILN |
| 4 | Concord Rangers | 2 | v | 2 | Biggleswade FC | 4 | 2-4 | | 4. ILN | 4. S1C |
| 4 | Lowestoft Town | 2 | v | 0 | Leverstock Green | 4 | | | 4. ILN | 4. S1C |
| 4 | Redbridge | 1 | v | 4 | Waltham Abbey | 4 | | | 4. ILN | 4. ILN |
| 4 | London Lions | 0 | v | 3 | Tilbury | 4 | | | 4. S1C | 4. ILN |
| 4 | Brightlingsea Regent | 0 | v | 3 | Leighton Town | 4 | | | 4. ILN | 4. S1C |
| 4 | Bowers & Pitsea | 0 | v | 3 | Cambridge City | 4 | | | 4. ILN | 4. ILN |
| 4 | Kingstonian | 1 | v | 0 | Merstham | 4 | | | 4. ISC | 4. ISE |
| 4 | Jersey Bulls | 3 | v | 1 | Hastings United | 4 | | | 4. ISE | 4. ISE |
| 4 | Leatherhead | 4 | v | 0 | Raynes Park Vale | 4 | | | 4. ISC | 4. ISC |
| 4 | Harrow Borough | 1 | v | 0 | Eastbourne Town | 4 | | | 4. ISC | 4. ISE |
| 4 | Hassocks | 1 | v | 3 | Crowborough Athletic | 4 | | | 4. ISE | 4. ISE |
| 4 | VCD Athletic | 0 | v | 0 | Rayners Lane | 4 | 5-4 | | 4. ISE | 4. S1C |
| 4 | Moneyfields | 2 | v | 2 | Thame United | 4 | 4-5 | | 4. ISC | 4. S1C |
| 4 | Herne Bay | 0 | v | 3 | Ashford United | 4 | | | 4. ISE | 4. ISE |
| 4 | Erith Town | 1 | v | 1 | Deal Town | 4 | 3-4 | | 4. ISE | 4. ISE |
| 4 | Bedfont Sports | 0 | v | 0 | Horndean | 4 | 4-2 | | 4. ISC | 4. ISC |
| 4 | Fareham Town | 4 | v | 1 | Beaconsfield Town | 4 | | | 4. ISC | 4. S1C |
| 4 | East Grinstead Town | 3 | v | 3 | Binfield | 4 | 4-2 | | 4. ISE | 4. ISC |
| 4 | South Park (Reigate) | 1 | v | 2 | Broadbridge Heath | 4 | | | 4. ISC | 4. ISE |
| 4 | Sittingbourne | 2 | v | 1 | Faversham Town | 4 | | | 4. ISE | 4. ISE |
| 4 | Westfield | 4 | v | 2 | Margate | 4 | | | 4. ISC | 4. ISE |
| 4 | Northwood | 0 | v | 1 | Beckenham Town | 4 | | | 4. S1C | 4. ISE |
| 4 | AFC Portchester | 1 | v | 0 | Flackwell Heath | 4 | | | 4. ISC | 4. S1C |
| 4 | Hayes & Yeading United | 1 | v | 0 | Metropolitan Police | 4 | | | 4. ISC | 4. ISC |
| 4 | Brixham | 0 | v | 4 | Falmouth Town | 4 | | | 4. S1S | 4. S1S |
| 4 | Tavistock AFC | 1 | v | 1 | Larkhall Athletic | 4 | 7-6 | | 4. S1S | 4. S1S |
| 4 | Bristol Manor Farm | 0 | v | 2 | Shaftesbury | 4 | | | 4. S1S | 4. S1S |
| 4 | Hartpury University | 2 | v | 1 | Didcot Town | 4 | | | 4. S1S | 4. S1S |
| 4 | Willand Rovers | 1 | v | 1 | Bishops Cleeve | 4 | 4-5 | | 4. S1S | 4. S1S |
| 4 | Bideford | 3 | v | 1 | Mousehole AFC | 4 | | | 4. S1S | 4. S1S |
| 4 | Exmouth Town | 1 | v | 1 | Swindon Supermarine | 4 | | | 4. S1S | 4. S1S |
| 4 | Winchester City | 2 | v | 0 | Frome Town | 4 | | | 4. S1S | 4. S1S |
| 4 | Portishead Town | 4 | v | 0 | Melksham Town | 4 | | | 4. S1S | 4. S1S |
| Sun Sep 7, 2025 |
| 4 | Pontefract Collieries | 0 | v | 2 | Lower Breck | 4 | | | 4. NPE | 4. NPW |
| 4 | Southall | 0 | v | 0 | AFC Whyteleafe | 4 | 5-4 | | 4. ISC | 4. ISE |
| 4 | Bognor Regis Town | 2 | v | 2 | Sevenoaks Town | 4 | 8-7 | | 4. ISC | 4. ISE |
| 4 | Littlehampton Town | 1 | v | 2 | Hendon | 4 | | | 4. ISC | 4. ISC |
| 2nd Qualifying Round |
| Fri Sep 19, 2025 |
| 4 | Stalybridge Celtic | 0 | v | 3 | Lower Breck | 4 | | | 4. NPW | 4. NPW |
| 4 | Bridlington Town | 2 | v | 0 | North Ferriby | 4 | | | 4. NPE | 4. NPE |
| 4 | Biggleswade FC | 3 | v | 1 | Hitchin Town | 4 | | | 4. S1C | 4. S1C |
| 4 | Enfield | 1 | v | 3 | AFC Dunstable | 4 | | | 4. S1C | 4. S1C |
| Sat Sep 20, 2025 |
| 4 | AFC Portchester | 3 | v | 0 | Tavistock AFC | 4 | | | 4. ISC | 4. S1S |
| 4 | Beckenham Town | 2 | v | 5 | Hendon | 4 | | | 4. ISE | 4. ISC |
| 4 | Bedworth United | 0 | v | 3 | Anstey Nomads | 4 | | | 4. NPM | 4. NPM |
| 4 | Bradford (Park Avenue) | 2 | v | 0 | Consett | 4 | | | 4. NPE | 4. NPE |
| 4 | Broadbridge Heath | 2 | v | 1 | Bedfont Sports | 4 | | | 4. ISE | 4. ISC |
| 4 | Cambridge City | 3 | v | 3 | Witham Town | 4 | 4-5 | | 4. ILN | 4. ILN |
| 4 | Coleshill Town | 3 | v | 4 | AFC Rushden & Diamonds | 4 | | | 4. NPM | 4. NPM |
| 4 | Congleton Town | 1 | v | 3 | Clitheroe | 4 | | | 4. NPW | 4. NPW |
| 4 | Corby Town | 3 | v | 0 | Sutton Coldfield Town | 4 | | | 4. NPM | 4. NPM |
| 4 | Crowborough Athletic | 1 | v | 6 | Sittingbourne | 4 | | | 4. ISE | 4. ISE |
| 4 | Deal Town | 2 | v | 3 | Ashford United | 4 | | | 4. ISE | 4. ISE |
| 4 | East Grinstead Town | 1 | v | 2 | VCD Athletic | 4 | | | 4. ISE | 4. ISE |
| 4 | Fareham Town | 2 | v | 4 | Bishops Cleeve | 4 | | | 4. ISC | 4. S1S |
| 4 | Grays Athletic | 2 | v | 0 | Felixstowe & Walton United | 4 | | | 4. ILN | 4. ILN |
| 4 | Hadley | 2 | v | 2 | Ascot United | 4 | 7-6 | | 4. S1C | 4. ISC |
| 4 | Harrow Borough | 4 | v | 0 | Jersey Bulls | 4 | | | 4. ISC | 4. ISE |
| 4 | Hartpury University | 4 | v | 2 | Portishead Town | 4 | | | 4. S1S | 4. S1S |
| 4 | Hayes & Yeading United | 5 | v | 1 | Southall | 4 | | | 4. ISC | 4. ISC |
| 4 | Heaton Stannington | 4 | v | 0 | Newton Aycliffe | 4 | | | 4. NPE | 4. NPE |
| 4 | Leatherhead | 2 | v | 0 | Kingstonian | 4 | | | 4. ISC | 4. ISC |
| 4 | Lincoln United | 0 | v | 2 | Malvern Town | 4 | | | 4. NPE | 4. S1S |
| 4 | Matlock Town | 1 | v | 2 | Basford United | 4 | | | 4. NPE | 4. NPM |
| 4 | Rugby Town | 3 | v | 2 | Belper Town | 4 | | | 4. NPM | 4. NPM |
| 4 | Shaftesbury | 2 | v | 2 | Falmouth Town | 4 | 4-1 | | 4. S1S | 4. S1S |
| 4 | Shifnal Town | 2 | v | 1 | Carlton Town | 4 | | | 4. NPW | 4. NPM |
| 4 | Silsden | 3 | v | 0 | Nantwich Town | 4 | | | 4. NPE | 4. NPW |
| 4 | Sporting Club Inkberrow | 4 | v | 3 | Stafford Rangers | 4 | | | 4. S1S | 4. NPW |
| 4 | Stanway Rovers | 3 | v | 0 | Mildenhall Town | 4 | | | 4. ILN | 4. ILN |
| 4 | Swindon Supermarine | 1 | v | 1 | Bideford | 4 | 4-5 | | 4. S1S | 4. S1S |
| 4 | Thame United | 3 | v | 1 | Winchester City | 4 | | | 4. S1C | 4. S1S |
| 4 | Waltham Abbey | 4 | v | 1 | Lowestoft Town | 4 | | | 4. ILN | 4. ILN |
| 4 | Walthamstow | 5 | v | 3 | Leighton Town | 4 | | | 4. ILN | 4. S1C |
| 4 | Welwyn Garden City | 2 | v | 2 | Tilbury | 4 | 3-4 | | 4. S1C | 4. ILN |
| 4 | Westfield | 1 | v | 1 | Bognor Regis Town | 4 | 4-3 | | 4. ISC | 4. ISC |
| 4 | Witton Albion | 0 | v | 1 | Dunston UTS | 4 | | | 4. NPW | 4. NPE |
| Sat Oct 4, 2025 |
| 4 | Runcorn Linnets | 7 | v | 0 | Bury | 4 | | | 4. NPW | 4. NPW |
| 3rd Qualifying Round |
| Fri Oct 3, 2025 |
| 4 | Bradford (Park Avenue) | 2 | v | 1 | Heaton Stannington | 4 | | | 4. NPE | 4. NPE |
| 3 | Worcester City | 2 | v | 2 | Rugby Town | 4 | 3-0 | | 3. SPC | 4. NPM |
| Sat Oct 4, 2025 |
| 4 | AFC Dunstable | 1 | v | 3 | Folkestone Invicta | 3 | | | 4. S1C | 3. IPL |
| 4 | AFC Portchester | 2 | v | 1 | Taunton Town | 3 | | Yes | 4. ISC | 3. SPS |
| 4 | AFC Rushden & Diamonds | 3 | v | 1 | Malvern Town | 4 | | | 4. NPM | 4. S1S |
| 3 | AFC Sudbury | 4 | v | 0 | Bury Town | 3 | | | 3. SPC | 3. SPC |
| 4 | Anstey Nomads | 3 | v | 2 | Stourbridge | 3 | | Yes | 4. NPM | 3. SPC |
| 4 | Ashford United | 3 | v | 3 | Biggleswade FC | 4 | 4-2 | | 4. ISE | 4. S1C |
| 3 | Ashton United | 1 | v | 2 | Clitheroe | 4 | | Yes | 3. NPL | 4. NPW |
| 3 | Aveley | 0 | v | 2 | St. Albans City | 3 | | | 3. IPL | 3. IPL |
| 3 | Barwell | 0 | v | 3 | Bromsgrove Sporting | 3 | | | 3. SPC | 3. SPC |
| 4 | Basford United | 0 | v | 0 | Alvechurch | 3 | 4-5 | | 4. NPM | 3. SPC |
| 3 | Basingstoke Town | 2 | v | 1 | Shaftesbury | 4 | | | 3. SPS | 4. S1S |
| 4 | Bishops Cleeve | 2 | v | 1 | Sholing | 3 | | Yes | 4. S1S | 3. SPS |
| 3 | Bishops Stortford | 4 | v | 0 | Cray Wanderers | 3 | | | 3. SPC | 3. IPL |
| 3 | Bracknell Town | 6 | v | 3 | Hungerford Town | 3 | | | 3. SPS | 3. SPS |
| 4 | Bridlington Town | 2 | v | 4 | Guiseley | 3 | | | 4. NPE | 3. NPL |
| 4 | Broadbridge Heath | 1 | v | 3 | Billericay Town | 3 | | | 4. ISE | 3. IPL |
| 3 | Burgess Hill Town | 1 | v | 1 | Potters Bar Town | 3 | 4-3 | | 3. IPL | 3. IPL |
| 3 | Carshalton Athletic | 1 | v | 2 | Cheshunt | 3 | | | 3. IPL | 3. IPL |
| 3 | Chatham Town | 0 | v | 0 | Needham Market | 3 | 3-2 | | 3. IPL | 3. SPC |
| 3 | Chertsey Town | 1 | v | 2 | Tilbury | 4 | | Yes | 3. SPS | 4. ILN |
| 3 | Chichester City | 2 | v | 4 | Farnham Town | 3 | | | 3. IPL | 3. SPS |
| 3 | Cleethorpes Town | 0 | v | 0 | Redditch United | 3 | 4-5 | | 3. NPL | 3. SPC |
| 3 | Cray Valley (Paper Mills) | 1 | v | 0 | Berkhamsted | 3 | | | 3. IPL | 3. SPS |
| 3 | Dorchester Town | 4 | v | 0 | Bideford | 4 | | | 3. SPS | 4. S1S |
| 3 | Dulwich Hamlet | 0 | v | 1 | Hendon | 4 | | Yes | 3. IPL | 4. ISC |
| 4 | Dunston UTS | 2 | v | 1 | Warrington Rylands 1906 | 3 | | Yes | 4. NPE | 3. NPL |
| 3 | Evesham United | 0 | v | 2 | Ilkeston Town | 3 | | | 3. SPS | 3. NPL |
| 3 | Gainsborough Trinity | 3 | v | 2 | Shifnal Town | 4 | | | 3. NPL | 4. NPW |
| 4 | Hadley | 2 | v | 1 | Dartford | 3 | | Yes | 4. S1C | 3. IPL |
| 3 | Halesowen Town | 1 | v | 2 | Leek Town | 3 | | | 3. SPC | 3. NPL |
| 3 | Hanwell Town | 2 | v | 1 | Grays Athletic | 4 | | | 3. SPS | 4. ILN |
| 3 | Harborough Town | 2 | v | 0 | Hednesford Town | 3 | | | 3. SPC | 3. NPL |
| 4 | Harrow Borough | 3 | v | 1 | Witham Town | 4 | | | 4. ISC | 4. ILN |
| 4 | Hartpury University | 0 | v | 0 | Gloucester City | 3 | 4-5 | | 4. S1S | 3. SPS |
| 3 | Havant & Waterlooville | 4 | v | 2 | Tiverton Town | 3 | | | 3. SPS | 3. SPS |
| 4 | Hayes & Yeading United | 0 | v | 0 | Walton & Hersham | 3 | 3-4 | | 4. ISC | 3. SPS |
| 3 | Hyde United | 1 | v | 0 | Warrington Town | 3 | | | 3. NPL | 3. NPL |
| 4 | Leatherhead | 4 | v | 1 | Ramsgate | 3 | | Yes | 4. ISC | 3. IPL |
| 3 | Lewes | 1 | v | 2 | Leiston | 3 | | | 3. IPL | 3. SPC |
| 3 | Plymouth Parkway | 3 | v | 2 | Gosport Borough | 3 | | | 3. SPS | 3. SPS |
| 3 | Quorn | 2 | v | 2 | Corby Town | 4 | 3-5 | Yes | 3. SPC | 4. NPM |
| 3 | Real Bedford | 3 | v | 1 | Stamford | 3 | | | 3. SPC | 3. SPC |
| 3 | Royston Town | 0 | v | 3 | Welling United | 3 | | | 3. SPC | 3. IPL |
| 3 | Rushall Olympic | 0 | v | 2 | Spalding United | 3 | | | 3. NPL | 3. SPC |
| 4 | Sittingbourne | 1 | v | 0 | Hashtag United | 3 | | Yes | 4. ISE | 3. IPL |
| 4 | Sporting Club Inkberrow | 1 | v | 2 | Stratford Town | 3 | | | 4. S1S | 3. SPC |
| 3 | St. Ives Town | 1 | v | 0 | Kettering Town | 3 | | | 3. SPC | 3. SPC |
| 4 | Stanway Rovers | 0 | v | 0 | Uxbridge | 3 | 5-3 | Yes | 4. ILN | 3. SPS |
| 3 | Stockton Town | 2 | v | 0 | Lower Breck | 4 | | | 3. NPL | 4. NPW |
| 4 | Thame United | 0 | v | 2 | Banbury United | 3 | | | 4. S1C | 3. SPC |
| 4 | Waltham Abbey | 6 | v | 0 | Walthamstow | 4 | | | 4. ILN | 4. ILN |
| 4 | Westfield | 1 | v | 2 | Brentwood Town | 3 | | | 4. ISC | 3. IPL |
| 3 | Whitby Town | 1 | v | 2 | Hebburn Town | 3 | | | 3. NPL | 3. NPL |
| 3 | Whitehawk | 0 | v | 2 | Canvey Island | 3 | | | 3. IPL | 3. IPL |
| Widnes | | v | w/o | Bamber Bridge | 3 | | | 3. NPL | 3. NPL |
| 3 | Wimborne Town | 2 | v | 0 | Weymouth | 3 | | | 3. SPS | 3. SPS |
| 3 | Wingate & Finchley | 4 | v | 1 | VCD Athletic | 4 | | | 3. IPL | 4. ISE |
| 3 | Yate Town | 1 | v | 2 | Poole Town | 3 | | | 3. SPS | 3. SPS |
| Tue Oct 7, 2025 |
| 3 | Prescot Cables | 2 | v | 0 | Lancaster City | 3 | | | 3. NPL | 3. NPL |
| 4 | Silsden | 1 | v | 1 | Stocksbridge Park Steels | 3 | 4-5 | | 4. NPE | 3. NPL |
| 3 | Workington | 0 | v | 2 | FC United of Manchester | 3 | | | 3. NPL | 3. NPL |
| 4 | Runcorn Linnets | 1 | v | 1 | Morpeth Town | 3 | 1-3 | | 4. NPW | 3. NPL |
| 1st Round |
| Fri Oct 24, 2025 |
| 4 | Dunston UTS | 1 | v | 0 | Hebburn Town | 3 | | Yes | 4. NPE | 3. NPL |
| Sat Oct 25, 2025 |
| 3 | AFC Sudbury | 2 | v | 1 | Leiston | 3 | | | 3. SPC | 3. SPC |
| 3 | Alvechurch | 0 | v | 0 | Worcester City | 3 | 4-3 | | 3. SPC | 3. SPC |
| 4 | Anstey Nomads | 4 | v | 0 | Stratford Town | 3 | | Yes | 4. NPM | 3. SPC |
| 3 | Billericay Town | 0 | v | 5 | Bishops Stortford | 3 | | | 3. IPL | 3. SPC |
| 4 | Bradford (Park Avenue) | 1 | v | 1 | Morpeth Town | 3 | 4-2 | Yes | 4. NPE | 3. NPL |
| 3 | Brentwood Town | 3 | v | 0 | Welling United | 3 | | | 3. IPL | 3. IPL |
| 3 | Burgess Hill Town | 1 | v | 4 | Folkestone Invicta | 3 | | | 3. IPL | 3. IPL |
| 4 | Clitheroe | 4 | v | 2 | Stockton Town | 3 | | Yes | 4. NPW | 3. NPL |
| 3 | Farnham Town | 2 | v | 2 | Bishops Cleeve | 4 | 3-1 | | 3. SPS | 4. S1S |
| 3 | FC United of Manchester | 1 | v | 0 | Bamber Bridge | 3 | | | 3. NPL | 3. NPL |
| 3 | Gloucester City | 1 | v | 0 | Havant & Waterlooville | 3 | | | 3. SPS | 3. SPS |
| 3 | Harborough Town | 4 | v | 1 | AFC Rushden & Diamonds | 4 | | | 3. SPC | 4. NPM |
| 3 | Hyde United | 0 | v | 3 | Stocksbridge Park Steels | 3 | | | 3. NPL | 3. NPL |
| 3 | Ilkeston Town | 3 | v | 1 | St. Ives Town | 3 | | | 3. NPL | 3. SPC |
| 4 | Leatherhead | 1 | v | 1 | Canvey Island | 3 | 4-2 | Yes | 4. ISC | 3. IPL |
| 3 | Leek Town | 3 | v | 0 | Bromsgrove Sporting | 3 | | | 3. NPL | 3. SPC |
| 3 | Plymouth Parkway | 1 | v | 1 | Poole Town | 3 | 7-6 | | 3. SPS | 3. SPS |
| 3 | Prescot Cables | 2 | v | 1 | Guiseley | 3 | | | 3. NPL | 3. NPL |
| 3 | Real Bedford | 3 | v | 2 | Ashford United | 4 | | | 3. SPC | 4. ISE |
| 3 | Redditch United | 1 | v | 2 | Gainsborough Trinity | 3 | | | 3. SPC | 3. NPL |
| 4 | Sittingbourne | 2 | v | 1 | Cray Valley (Paper Mills) | 3 | | Yes | 4. ISE | 3. IPL |
| 3 | Spalding United | 0 | v | 1 | Corby Town | 4 | | Yes | 3. SPC | 4. NPM |
| 3 | St. Albans City | 0 | v | 2 | Hanwell Town | 3 | | | 3. IPL | 3. SPS |
| 4 | Stanway Rovers | 1 | v | 2 | Harrow Borough | 4 | | | 4. ILN | 4. ISC |
| 4 | Tilbury | 1 | v | 0 | Hadley | 4 | | | 4. ILN | 4. S1C |
| 4 | Waltham Abbey | 0 | v | 2 | Chatham Town | 3 | | | 4. ILN | 3. IPL |
| 3 | Walton & Hersham | 5 | v | 1 | Hendon | 4 | | | 3. SPS | 4. ISC |
| 3 | Wimborne Town | 1 | v | 0 | AFC Portchester | 4 | | | 3. SPS | 4. ISC |
| 3 | Wingate & Finchley | 0 | v | 1 | Cheshunt | 3 | | | 3. IPL | 3. IPL |
| Sun Oct 26, 2025 |
| 3 | Bracknell Town | 1 | v | 0 | Banbury United | 3 | | | 3. SPS | 3. SPC |
| Tue Oct 28, 2025 |
| 3 | Basingstoke Town | 1 | v | 1 | Dorchester Town | 3 | 4-5 | | 3. SPS | 3. SPS |
| 2nd Round |
| Sat Nov 15, 2025 |
| 3 | AFC Sudbury | 1 | v | 5 | Hereford | 2 | | | 3. SPC | 2. NLN |
| 3 | Alvechurch | 1 | v | 1 | Real Bedford | 3 | 4-3 | | 3. SPC | 3. SPC |
| 2 | Bath City | 1 | v | 1 | Bracknell Town | 3 | 5-3 | | 2. NLS | 3. SPS |
| 2 | Bedford Town | 0 | v | 1 | Anstey Nomads | 4 | | Yes | 2. NLN | 4. NPM |
| 3 | Bishops Stortford | 1 | v | 1 | Cheshunt | 3 | 4-5 | | 3. SPC | 3. IPL |
| 3 | Brentwood Town | 0 | v | 0 | Hornchurch | 2 | 2-4 | Yes | 3. IPL | 2. NLS |
| 2 | Buxton | 2 | v | 3 | Southport | 2 | | | 2. NLN | 2. NLN |
| 2 | Chesham United | 1 | v | 2 | AFC Telford United | 2 | | | 2. NLS | 2. NLN |
| 2 | Chester | 0 | v | 1 | Clitheroe | 4 | | Yes | 2. NLN | 4. NPW |
| 2 | Chippenham Town | 0 | v | 1 | Weston super Mare | 2 | | | 2. NLS | 2. NLS |
| 2 | Chorley | 2 | v | 3 | Worksop Town | 2 | | | 2. NLN | 2. NLN |
| 2 | Dagenham & Redbridge | 2 | v | 0 | Tilbury | 4 | | | 2. NLS | 4. ILN |
| 2 | Dorking Wanderers | 3 | v | 2 | Eastbourne Borough | 2 | | | 2. NLS | 2. NLS |
| 2 | Dover Athletic | 2 | v | 0 | Dorchester Town | 3 | | | 2. NLS | 3. SPS |
| 2 | Ebbsfleet United | 1 | v | 1 | Sittingbourne | 4 | 5-3 | | 2. NLS | 4. ISE |
| 3 | FC United of Manchester | 3 | v | 2 | Darlington | 2 | | Yes | 3. NPL | 2. NLN |
| 3 | Folkestone Invicta | 1 | v | 2 | AFC Totton | 2 | | | 3. IPL | 2. NLS |
| 3 | Harborough Town | 3 | v | 0 | Enfield Town | 2 | | Yes | 3. SPC | 2. NLS |
| 2 | Hemel Hempstead Town | 0 | v | 0 | Chelmsford City | 2 | 6-5 | | 2. NLS | 2. NLS |
| 2 | Horsham | 2 | v | 0 | Farnham Town | 3 | | | 2. NLS | 3. SPS |
| 3 | Ilkeston Town | 0 | v | 1 | Curzon Ashton | 2 | | | 3. NPL | 2. NLN |
| 2 | Kidderminster Harriers | 3 | v | 0 | Corby Town | 4 | | | 2. NLN | 4. NPM |
| 2 | Kings Lynn Town | 2 | v | 2 | Harrow Borough | 4 | 2-3 | Yes | 2. NLN | 4. ISC |
| 2 | Leamington | 2 | v | 0 | Maidenhead United | 2 | | | 2. NLN | 2. NLS |
| 4 | Leatherhead | 2 | v | 0 | Hampton & Richmond Borough | 2 | | Yes | 4. ISC | 2. NLS |
| 3 | Leek Town | 1 | v | 0 | Scarborough Athletic | 2 | | Yes | 3. NPL | 2. NLN |
| 2 | Macclesfield | 1 | v | 0 | Prescot Cables | 3 | | | 2. NLN | 3. NPL |
| 2 | Marine | 2 | v | 0 | Stocksbridge Park Steels | 3 | | | 2. NLN | 3. NPL |
| 2 | Merthyr Town | 2 | v | 3 | Hanwell Town | 3 | | Yes | 2. NLN | 3. SPS |
| 2 | Oxford City | 1 | v | 2 | Peterborough Sports | 2 | | | 2. NLN | 2. NLN |
| 3 | Plymouth Parkway | 4 | v | 3 | Salisbury | 2 | | Yes | 3. SPS | 2. NLS |
| 2 | Radcliffe | 2 | v | 2 | Bradford (Park Avenue) | 4 | 6-5 | | 2. NLN | 4. NPE |
| 2 | Slough Town | 1 | v | 1 | Gloucester City | 3 | 3-4 | | 2. NLS | 3. SPS |
| 2 | South Shields | 6 | v | 2 | Dunston UTS | 4 | | | 2. NLN | 4. NPE |
| 2 | Spennymoor Town | 2 | v | 3 | AFC Fylde | 2 | | | 2. NLN | 2. NLN |
| 2 | Tonbridge Angels | 0 | v | 4 | Worthing | 2 | | | 2. NLS | 2. NLS |
| 2 | Torquay United | 0 | v | 2 | Maidstone United | 2 | | | 2. NLS | 2. NLS |
| 3 | Walton & Hersham | 2 | v | 1 | Farnborough | 2 | | Yes | 3. SPS | 2. NLS |
| 3 | Wimborne Town | 0 | v | 1 | Chatham Town | 3 | | | 3. SPS | 3. IPL |
| Tue Nov 18, 2025 |
| 3 | Gainsborough Trinity | 1 | v | 2 | Alfreton Town | 2 | | | 3. NPL | 2. NLN |
| 3rd Round |
| Sat Dec 13, 2025 |
| 2 | AFC Telford United | 4 | v | 3 | Altrincham | 1 | | Yes | 2. NLN | 1. NLP |
| 3 | Alvechurch | 2 | v | 2 | Alfreton Town | 2 | 4-1 | Yes | 3. SPC | 2. NLN |
| 1 | Boreham Wood | 3 | v | 3 | Brackley Town | 1 | 1-3 | | 1. NLP | 1. NLP |
| 3 | Chatham Town | 3 | v | 0 | Plymouth Parkway | 3 | | | 3. IPL | 3. SPS |
| 4 | Clitheroe | 1 | v | 1 | York City | 1 | 4-3 | Yes | 4. NPW | 1. NLP |
| 2 | Curzon Ashton | 1 | v | 2 | Marine | 2 | | | 2. NLN | 2. NLN |
| 2 | Dagenham & Redbridge | 3 | v | 1 | Hanwell Town | 3 | | | 2. NLS | 3. SPS |
| 2 | Dorking Wanderers | 1 | v | 1 | Bath City | 2 | 2-4 | | 2. NLS | 2. NLS |
| 2 | Dover Athletic | 1 | v | 4 | Hornchurch | 2 | | | 2. NLS | 2. NLS |
| 1 | Eastleigh | 2 | v | 1 | Aldershot Town | 1 | | | 1. NLP | 1. NLP |
| 2 | Ebbsfleet United | 3 | v | 1 | Braintree Town | 1 | | Yes | 2. NLS | 1. NLP |
| 1 | FC Halifax Town | 4 | v | 0 | FC United of Manchester | 3 | | | 1. NLP | 3. NPL |
| 1 | Forest Green Rovers | 4 | v | 0 | Weston super Mare | 2 | | | 1. NLP | 2. NLS |
| 1 | Gateshead | 0 | v | 3 | Morecambe | 1 | | | 1. NLP | 1. NLP |
| 3 | Gloucester City | 3 | v | 3 | Worthing | 2 | 5-3 | Yes | 3. SPS | 2. NLS |
| 3 | Harborough Town | 1 | v | 2 | Carlisle United | 1 | | | 3. SPC | 1. NLP |
| 1 | Hartlepool United | 1 | v | 2 | Anstey Nomads | 4 | | Yes | 1. NLP | 4. NPM |
| 2 | Hemel Hempstead Town | 0 | v | 2 | Horsham | 2 | | | 2. NLS | 2. NLS |
| 2 | Hereford | 1 | v | 1 | Radcliffe | 2 | 4-2 | | 2. NLN | 2. NLN |
| 2 | Leamington | 0 | v | 1 | Rochdale | 1 | | | 2. NLN | 1. NLP |
| 4 | Leatherhead | 3 | v | 2 | Harrow Borough | 4 | | | 4. ISC | 4. ISC |
| 1 | Scunthorpe United | 5 | v | 0 | Peterborough Sports | 2 | | | 1. NLP | 2. NLN |
| 1 | Solihull Moors | 2 | v | 2 | AFC Fylde | 2 | 8-9 | Yes | 1. NLP | 2. NLN |
| 2 | South Shields | 0 | v | 1 | Macclesfield | 2 | | | 2. NLN | 2. NLN |
| 1 | Southend United | 4 | v | 0 | Truro City | 1 | | | 1. NLP | 1. NLP |
| 2 | Southport | 3 | v | 1 | Leek Town | 3 | | | 2. NLN | 3. NPL |
| 1 | Tamworth | 1 | v | 1 | Boston United | 1 | 5-4 | | 1. NLP | 1. NLP |
| 3 | Walton & Hersham | 4 | v | 1 | Sutton United | 1 | | Yes | 3. SPS | 1. NLP |
| 1 | Wealdstone | 2 | v | 1 | Cheshunt | 3 | | | 1. NLP | 3. IPL |
| 1 | Woking | 2 | v | 0 | AFC Totton | 2 | | | 1. NLP | 2. NLS |
| 2 | Worksop Town | 0 | v | 0 | Kidderminster Harriers | 2 | 1-4 | | 2. NLN | 2. NLN |
| 1 | Yeovil Town | 1 | v | 1 | Maidstone United | 2 | 4-2 | | 1. NLP | 2. NLS |
| 4th Round |
| Sat Jan 10, 2026 |
| 3 | Chatham Town | 1 | v | 0 | Carlisle United | 1 | | Yes | 3. IPL | 1. NLP |
| 2 | Ebbsfleet United | 0 | v | 2 | Forest Green Rovers | 1 | | | 2. NLS | 1. NLP |
| 2 | Hereford | 2 | v | 2 | AFC Fylde | 2 | 3-4 | | 2. NLN | 2. NLN |
| 4 | Leatherhead | 2 | v | 3 | Horsham | 2 | | | 4. ISC | 2. NLS |
| 2 | Marine | 1 | v | 1 | Gloucester City | 3 | 4-3 | | 2. NLN | 3. SPS |
| 3 | Walton & Hersham | 1 | v | 0 | Brackley Town | 1 | | Yes | 3. SPS | 1. NLP |
| 1 | Wealdstone | 1 | v | 0 | Dagenham & Redbridge | 2 | | | 1. NLP | 2. NLS |
| 1 | Yeovil Town | 0 | v | 0 | Alvechurch | 3 | 3-1 | | 1. NLP | 3. SPC |
| Tue Jan 13, 2026 |
| 2 | Kidderminster Harriers | 6 | v | 2 | Morecambe | 1 | | Yes | 2. NLN | 1. NLP |
| 1 | Scunthorpe United | 3 | v | 1 | Clitheroe | 4 | | | 1. NLP | 4. NPW |
| 1 | Tamworth | 1 | v | 1 | Rochdale | 1 | 7-6 | | 1. NLP | 1. NLP |
| 2 | Bath City | 1 | v | 3 | Southend United | 1 | | | 2. NLS | 1. NLP |
| 1 | FC Halifax Town | 4 | v | 0 | Anstey Nomads | 4 | | | 1. NLP | 4. NPM |
| 1 | Eastleigh | 0 | v | 2 | Southport | 2 | | Yes | 1. NLP | 2. NLN |
| Tue Jan 20, 2026 |
| 2 | Hornchurch | 1 | v | 2 | AFC Telford United | 2 | | | 2. NLS | 2. NLN |
| 1 | Woking | 2 | v | 1 | Macclesfield | 2 | | | 1. NLP | 2. NLN |
| 5th Round |
| Sat Jan 31, 2026 |
| 2 | AFC Fylde | 0 | v | 1 | Southport | 2 | | | 2. NLN | 2. NLN |
| 3 | Chatham Town | 0 | v | 1 | Southend United | 1 | | | 3. IPL | 1. NLP |
| 1 | FC Halifax Town | 1 | v | 1 | Kidderminster Harriers | 2 | 3-4 | Yes | 1. NLP | 2. NLN |
| 1 | Forest Green Rovers | 0 | v | 3 | Wealdstone | 1 | | | 1. NLP | 1. NLP |
| 2 | AFC Telford United | 0 | v | 2 | Yeovil Town | 1 | | | 2. NLN | 1. NLP |
| 1 | Scunthorpe United | 1 | v | 2 | Horsham | 2 | | Yes | 1. NLP | 2. NLS |
| 1 | Tamworth | 0 | v | 0 | Marine | 2 | 1-4 | Yes | 1. NLP | 2. NLN |
| 3 | Walton & Hersham | 1 | v | 2 | Woking | 1 | | | 3. SPS | 1. NLP |
| Quarter Final |
| Sat Feb 28, 2026 |
| 2 | Southport | | v | | Yeovil Town | 1 | | | 2. NLN | 1. NLP |
| 2 | Marine | | v | | Woking | 1 | | | 2. NLN | 1. NLP |
| 2 | Kidderminster Harriers | | v | | Wealdstone | 1 | | | 2. NLN | 1. NLP |
| 2 | Horsham | | v | | Southend United | 1 | | | 2. NLS | 1. NLP |
